Research Scientist/Engineer 1 (Temporary)
Req #: | 173163 |
Department: | Pathology |
Appointing Department Web Address: | http://www.pathology.washington.edu/ |
Job Location: | South Lake Union |
Posting Date: | 10/15/2019 |
Closing Info: | Open Until Filled |
Salary: | Salary is Competitive. Salary is Commensurate Upon Qualifications and Experience. |
Shift: | First Shift |
The University of Washington’s Department of Pathology is a broad and dynamic department that strives for a consistent level of high achievement in all of its activities. The Department offers full diagnostic services in all areas of Pathology, is a top ranked research organization as measured by research awards from the National Institutes of Health, and offers an array of specialized educational training programs.
UW Medicine Pathology brings together state-of-the-art technology and highly trained, world renowned personnel to provide comprehensive diagnostic and personalized service to physicians, community hospitals, independent laboratories, and other health care organizations. Our operations are based at the University of Washington Medical Center, Harborview Medical Center, South Lake Union, Northwest Hospital and the Seattle Cancer Care Alliance. Pathology faculty members also serve at Seattle Children's Hospital, the Veteran’s Administration Puget Sound Healthcare System, and the Fred Hutchinson Cancer Research Center. These collaborative efforts have generated innovative, translational studies in the basic mechanisms of disease as they apply to diagnostic pathology.
The Heart Engineering and Developmental Genomics laboratory in the Department of Pathology at the University of Washington is seeking for a motivated Temporary Research Scientist/Engineer I (RSE-I). This position will be a full time, temporary appointment under the joint supervision of Drs. Alessandro Bertero and Charles E. Murry.
This RSE-I will join a research group with the following goals: (1) to elucidate the regulatory mechanisms underpinning cardiac development; and (2) to leverage this knowledge to develop gene, cell, and tissue engineering strategies for cardiac therapeutics. The current focus of developmental genomics studies is the role of three-dimensional chromatin organization in normal and abnormal cardiac development. Engineering efforts are now concentrated on the generation of genetically modified human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) to improve both their safety and efficacy in cardiac regenerative medicine applications.
The job responsibilities for this position will include: (1) the generation of targeting constructs and CRISPR/Cas9 plasmids for gene editing experiments; (2) the delivery of these regents to hPSCs by transfection or nucleofection; (3) clonal selection, genotyping, quality control, and banking of of gene-edited lines; and (4) differentiation, quality control, and banking of hPSC-CMs. The RSE-I will maintain accurate experimental records and reagent inventories, analyze results, and present their work during regular lab meetings. Moreover, the RSE-I will assist the routine operations of the laboratory, including ordering, inventory management, supervision of undergraduate student helpers, and preparation of materials and reagents.
Candidates for this position will hold a Bachelor’s degree in biochemistry or a related field, and will have at least two years of prior relevant experience (some or all during an educational program). In particular, the ideal candidate will have prior expertise in hPSC culture, hPSC-CM differentiation, transfection, PCR, western blot, and flow cytometry. Other desired skills include laboratory inventory management, proficiency with the usage of Microsoft Office, and efficient oral and written communication.
Under the general guidance of Dr. Bertero, the RSE-I will be required to work under limited direct supervision to achieve defined research goals using established protocols and techniques. Training will be provided for new aspects and assignments. The candidate should be open to flexible working hours involving occasional weekend activities as required to maintain hPSC and hPSC-CM cultures.
Requirements
• B.S. in Biochemistry or related field
• At least two years of experience in cell culture and molecular biology
• Expertise in transfection, PCR, western blot, and flow cytometry
• Strong communication and organizational skills
Desired
• Familiarity with culture of human pluripotent stem cells (hPSCs)
• Familiarity with differentiation of hPSCs into cardiomyocytes
• Experience in laboratory management.
Application Process:
The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter, and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.
The University of Washington is a leader in environmental stewardship & sustainability, and committed to becoming climate neutral.
The University of Washington is an equal opportunity, affirmative action employer. To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 / 206-543-6452 (tty) or dso@uw.edu.