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RESEARCH SCIENTIST/ENGINEER 1

University of Washington
Seattle, WA Full-time
Posted on November 30, 2019
Req #: 166220
Department: BIOCHEMISTRY
Job Location: Seattle Campus
Posting Date: 04/03/2019 
Closing Info: Open Until Filled 
Salary: Salary is commensurate with education and experience 
Shift: First Shift 
Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here.  

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. All of which has allowed the UW to be nationally recognized as a “Great College to Work For” for four consecutive years.

The Department of Biochemistry at the University of Washington has contributed to many phases of research. Initially recognized for incisive studies of the structure and function of proteins, and the mechanisms of enzyme action, the department branched out into a multitude of other areas. These included: developmental biology of flies, fish, frogs, and mice; molecular mechanisms of aging in yeast and mammals; vertebrate visual and immune responses; chromosome dynamics, cell division, DNA replication and repair; energy transduction during photosynthesis; protein secretion and membrane fusion; translational control and regulated protein degradation; protein structure determination by crystallography and NMR spectroscopy; and protein structure prediction and enzyme design. Our diversity of interests guarantees a rich intellectual and educational environment.

A laboratory in the UW Department of Biochemistry currently has an outstanding opportunity for a Research Scientist/Engineer 1 for studies of membrane protein function and structure. The overall project combines high-throughput saturation mutagenesis approaches with structural analyses and biochemical reconstitution to understand the mechanisms of membrane docking and fusion.

Responsibilities::

The Research Scientist/Engineer will:
  • Clone and purify proteins from E. coli, S. cerevisiae, and other microbes, using affinity and classical chromatography techniques.
  • Perform biochemical reconstitution experiments to test the functions of membrane-resident proteins, using fluorescence (FRET and anisotropy) techniques
  • Structurally characterize these protein complexes using electron cryo microscopy and mass spectrometry approaches.

    Minimum Requirements:
  • Bachelor’s degree in Chemistry, Biochemistry, Biomedical Engineering, or similar discipline, with at least one year of prior laboratory research experience.

    Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.

    Other required qualifications include:
  • Strong organizational skills.
  • Ability and ambition to learn new techniques and approaches.
  • Strong quantitative skills, with computer programming experience preferred.
  • Ability and enthusiasm to work as part of a team.
    •Intellectual integrity.

    Desired:
  • Experience with protein expression and purification including liquid chromatography.
  • Practical understanding of recombinant DNA and cloning strategies and tactics, including but not limited to polycistronic vector design and Gibson cloning.
  • Experience with PyMol or Chimera molecular modeling packages. Experience using Python and R languages in a life science setting.
  • Ability to learn and integrate novel wet bench and in silico methods.

    Application Process:
    The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process.  These assessments may include Work Authorization, Cover Letter and/or others.  Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page.  If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
  • Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.

    The University of Washington is a leader in environmental stewardship & sustainability, and committed to becoming climate neutral.

    The University of Washington is an equal opportunity, affirmative action employer. To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 / 206-543-6452 (tty) or dso@uw.edu.